Alcohol binge disrupts the rat intestinal barrier: the partial protective role of oleoylethanolamide.

ABSTRACT:

Background and purpose

Chronic alcohol consumption alters the gut-brain axis, but little is known about alcohol binge episodes on the functioning of the intestinal barrier. We investigated the influence of ethanol binges on bacterial translocation, gut inflammation and immunity, and tight junction (TJ) structure and the ability of the biolipid oleoylethanolamide (OEA) to prevent ethanol binge-induced intestinal barrier dysfunction.

Experimental approach

OEA was injected i.p. before repeated ethanol administration by oral gavage. Plasma, spleen, liver and mesenteric lymph nodes (MLN) were collected in sterile conditions for determination of bacterial load. Immune/inflammatory parameters, TJ proteins and apoptotic markers were determined in colonic tissue by RT-PCR and Western

SUBMITTER: Anton M 

PROVIDER: S-EPMC6255955 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature