Reduction in Methane Emissions From Acidified Dairy Slurry Is Related to Inhibition of Methanosarcina Species.

ABSTRACT: Liquid dairy manure treated with sulfuric acid was stored in duplicate pilot-scale storage tanks for 120 days with continuous monitoring of CH₄ emissions and concurrent examination of changes in the structure of bacterial and methanogenic communities. Methane emissions were monitored at the site using laser-based Trace Gas Analyzer whereas quantitative real-time polymerase chain reaction and massively parallel sequencing were employed to study bacterial and methanogenic communities using 16S rRNA and methyl-coenzyme M Reductase A (mcrA) genes/transcripts, respectively. When compared with untreated slurries, acidification resulted in 69-84% reductions of cumulative CH₄ emissions. The abundance, activity, and proportion of bacterial communities did not vary with

SUBMITTER: Habtewold J 

PROVIDER: S-EPMC6255968 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature