Food-effect study on uracil and dihydrouracil plasma levels as marker for dihydropyrimidine dehydrogenase activity in human volunteers.

ABSTRACT:

Aims

This study aimed to determine the effect of food intake on uracil and dihydrouracil plasma levels. These levels are a promising marker for dihydropyrimidine dehydrogenase activity and for individualizing fluoropyrimidine anticancer therapy.

Methods

A randomized, cross-over study in 16 healthy volunteers was performed, in which subjects were examined in fasted and fed state on two separate days. In fed condition, a high-fat, high-caloric breakfast was consumed between 8:00 h and 8:30 h. Whole blood for determination of uracil, dihydrouracil and uridine plasma levels was drawn on both test days at predefined time points between 8:00 h and 13:00 h.

Results

Uracil levels were statistically significantly different between fasting and fed state. At 13:00 h, the mean ur

SUBMITTER: Henricks LM 

PROVIDER: S-EPMC6256055 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature