Facile chemoenzymatic synthesis of a novel stable mimic of NAD.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) is an essential cofactor participating in a variety of important enzyme-catalyzed physiological and pathophysiological processes. Analogues of NAD⁺ provide key and valuable agents for investigating NAD⁺-dependent enzymes. In this study, we report the preparation of a novel stable NAD⁺ mimic, 4'-thioribose NAD⁺ (S-NAD⁺), using a facile and efficient chemoenzymatic approach. Substrate activity assays indicated the resulting S-NAD⁺ is chemically inert to human CD38 and sirtuin 2 enzymes, but capable of participating in redox reactions in a manner similar to NAD⁺. X-ray crystallographic analysis revealed binding of S-NAD⁺ to the active site of human CD38 a

SUBMITTER: Dai Z 

PROVIDER: S-EPMC6256357 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature