MiR-671-5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP.

ABSTRACT: Various studies have demonstrated that microRNA (miRNA) serves an important role in the development of gastric cancer. However, the expression level, clinical significance and the biological function of miRNA in gastric cancer remain largely unknown. The present study investigated the exact roles of miR-671-5p in gastric cancer, confirmed its target and explored its mechanism. Initially, the low expression levels of miR-671-5p in gastric cancer cells were confirmed by reverse transcription-quantitative polymerase chain reaction. TargetScan and MiRanda databases were utilized to forecast the target genes of miR-671-5p, and the prediction was verified by dual-luciferase reporter assay and western blot analysis. Cell Counting Kit-8 was used for cell proliferation detection. An annexin V-fluor

SUBMITTER: Qiu T 

PROVIDER: S-EPMC6256858 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature