MiRNA-299-5p regulates estrogen receptor alpha and inhibits migration and invasion of papillary thyroid cancer cell.

ABSTRACT:

Background

The incidence of papillary thyroid cancer (PTC) is increasing faster than any other solid tumors worldwide. Invasion and metastasis are the main reasons for the poor prognosis of patients with PTC. Previously, we observed significantly low expression of miRNA-299-5p in invasive PTC tissue samples.

Aim

The present study aimed to determine whether miR-299-5p plays a key role in PTC migration and invasion.

Materials and methods

The miR-299-5p expression level was measured using quantitative real-time PCR in 109 human PTC samples and paired adjacent normal tissues and in the human BCPAP PTC cell line. The effects of miR-299-5p on PTC cell migration and invasion were assessed using wound healing and transwell assays. In addition, we searched for the miR-299-5p t

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6257197 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature