Migration through a small pore disrupts inactive chromatin organization in neutrophil-like cells.

ABSTRACT:

Background

Mammalian cells are flexible and can rapidly change shape when they contract, adhere, or migrate. The nucleus must be stiff enough to withstand cytoskeletal forces, but flexible enough to remodel as the cell changes shape. This is particularly important for cells migrating through confined spaces, where the nuclear shape must change in order to fit through a constriction. This occurs many times in the life cycle of a neutrophil, which must protect its chromatin from damage and disruption associated with migration. Here we characterized the effects of constricted migration in neutrophil-like cells.

Results

Total RNA sequencing identified that migration of neutrophil-like cells through 5- or 14-μm pores was associated with changes in the transcript levels of inflamm

SUBMITTER: Jacobson EC 

PROVIDER: S-EPMC6257957 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature