Stable integration of the Mrx1-roGFP2 biosensor to monitor dynamic changes of the mycothiol redox potential in Corynebacterium glutamicum.

ABSTRACT: Mycothiol (MSH) functions as major low molecular weight (LMW) thiol in the industrially important Corynebacterium glutamicum. In this study, we genomically integrated an Mrx1-roGFP2 biosensor in C. glutamicum to measure dynamic changes of the MSH redox potential (E_MSH) during the growth and under oxidative stress. C. glutamicum maintains a highly reducing intrabacterial E_MSH throughout the growth curve with basal E_MSH levels of ~- 296 mV. Consistent with its H₂O₂ resistant phenotype, C. glutamicum responds only weakly to 40 mM H₂O₂, but is rapidly oxidized by low doses of NaOCl. We further monitored basal E_MSH changes and the H₂O₂ response in various mutants which are compromised i

SUBMITTER: Tung QN 

PROVIDER: S-EPMC6258114 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature