Comprehensive proteome analyses of lysine acetylation in tea leaves by sensing nitrogen nutrition.

ABSTRACT:

Background

N^ε-Acetylation of lysine residues, a frequently occurring post-translational modification, plays important functions in regulating physiology and metabolism. However, the information of global overview of protein acetylome under nitrogen-starvation/resupply in tea (Camellia sinensis) leaves was limited. And the full function of lysine acetylated proteins of tea plants in nitrogen absorption and assimilation remains unclear.

Results

Here, we performed the global review of lysine acetylome in tea leaves under nitrogen (N)-starvation/resupply, using peptide prefractionation, immunoaffinity enrichment, and coupling with high sensitive LC-MS/MS combined with affinity purification analysis. Altogether, 2229 lysine acetylation sites on 1286 proteins were ident

SUBMITTER: Jiang J 

PROVIDER: S-EPMC6258439 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature