Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell.

ABSTRACT: Lentiviral vectors (LVs) have recently witnessed an increasing demand in research and clinical applications. Their current purification processes represent the main bottleneck in their widespread use, as the methods used are cumbersome and yield low recoveries. We aimed to develop a one-step method to specifically purify LVs, with high yields and reduced levels of impurities, using the biotin-streptavidin system. Herein, packaging HEK293T cells were genetically engineered with a cyclical biotin-mimicking peptide displayed on a CD8α stalk, termed cTag8. LVs were modified with cTag8 by its passive incorporation onto viral surfaces during budding, without viral protein engineering or hindrance on infectivity. Expression of cTag8 on LVs allowed complete capture of infectious particles by strep

SUBMITTER: Mekkaoui L 

PROVIDER: S-EPMC6258877 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature