Identification and application of a growth-regulated promoter for improving L-valine production in Corynebacterium glutamicum.

ABSTRACT:

Background

Promoters are commonly used to regulate the expression of specific target genes or operons. Although a series of promoters have been developed in Corynebacterium glutamicum, more precise and unique expression patterns are needed that the current selection of promoters cannot produce. RNA-Seq technology is a powerful tool for helping us to screen out promoters with expected transcriptional strengths.

Results

The promoter P_{CP_2836} of an aldehyde dehydrogenase coding gene from Corynebacterium glutamicum CP was identified via RNA-seq and RT-PCR as a growth-regulated promoter. Comparing with the strong constitutive promoter P_tuf, the transcriptional strength of P_{CP_2836} showed a significant decrease that from about 75 to 8% in the stati

SUBMITTER: Ma Y 

PROVIDER: S-EPMC6260661 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature