Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D.

ABSTRACT:

Background

Drugs that inhibit the MEK/ERK pathway have therapeutic benefit in bladder cancer treatment but responses vary with patients, for reasons that are still not very clear. Interferon-α (IFN-α) is also used as a therapeutic agent for bladder cancer treatment but the response rate is low. It was found that IFN-α could enhance the cytotoxic effect of MEK inhibition. However, the potential mechanisms of that are still unclear. Understanding of the cross-talk between the IFN-α and MEK/ERK pathway will help enhance the efficacy of IFN-α or MEK inhibitors on bladder cancer.

Methods

Immunoprecipitation and pull-down assay were used to reveal the formation of signaling complex. The protein expressions were detected by western blot and immunohistochemistry. The cAMP level, Pho

SUBMITTER: Qiang Z 

PROVIDER: S-EPMC6260752 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature