Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.

ABSTRACT:

Background

The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can be physiologically impaired and show tendencies for cellular agglomeration, hence are hard to cultivate. Further, comprehensive data regarding growth, physiology and recombinant protein production in the controlled environment of a bioreactor are scarce.

Results

A Man₅GlcNAc₂ glycosylating and a Man_8-10GlcNAc₂ glycosylating strain showed similar morphological traits during methanol induced shake-flask cultivations to

SUBMITTER: Pekarsky A 

PROVIDER: S-EPMC6260843 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature