Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent.

ABSTRACT:

Background

The viral or host systems for a gene expression assume repeatability of the process and high quality of the protein product. Since level and fidelity of transcription primarily determines the overall efficiency, all factors contributing to their decrease should be identified and optimized. Among many observed processes, non-programmed insertion/deletion (indel) of nucleotide during transcription (slippage) occurring at homopolymeric A/T sequences within a gene can considerably impact its expression. To date, no comparative study of the most utilized Escherichia coli and T7 bacteriophage RNA polymerases (RNAP) propensity for this type of erroneous mRNA synthesis has been reported. To address this issue we evaluated the influence of shift-prone A/T sequences by assessing i

SUBMITTER: Koscielniak D 

PROVIDER: S-EPMC6260861 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature