MiR-181b/Notch2 overcome chemoresistance by regulating cancer stem cell-like properties in NSCLC.

ABSTRACT:

Background

Lung cancer stem cells have the ability to self-renew and are resistant to conventional chemotherapy. MicroRNAs (miRNAs) regulate and control the expression and function of many target genes; therefore, miRNA disorders are involved in the pathogenesis of human diseases, such as cancer. However, the effects of miRNA dysregulation on tumour stemness and drug resistance have not been fully elucidated. miR-181b has been reported to be a tumour suppressor miRNA and is associated with drug-resistant non-small cell lung cancer.

Methods

Cancer stem cell (CSC)-like properties were tested by a cell proliferation assay and flow cytometry; miR-181b expression was measured by real-time PCR; and Notch2 and related proteins were detected by Western blotting and immunohistochemis

SUBMITTER: Wang X 

PROVIDER: S-EPMC6260863 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature