NMR-Guided Mass Spectrometry for Absolute Quantitation of Human Blood Metabolites.

ABSTRACT: Broad-based, targeted metabolite profiling using mass spectrometry (MS) has become a major platform used in the field of metabolomics for a variety of applications. However, quantitative MS analysis is challenging owing to numerous factors including (1) the need for, ideally, isotope-labeled internal standards for each metabolite, (2) the fact that such standards may be unavailable or prohibitively costly, (3) the need to maintain the standards' concentrations close to those of the target metabolites, and (4) the alternative use of time-consuming calibration curves for each target metabolite. Here, we introduce a new method in which metabolites from a single serum specimen are quantified on the basis of a recently developed NMR method [ Nagana Gowda et al. Anal. Chem. 2015 , 87 , 706 ] and

SUBMITTER: Nagana Gowda GA 

PROVIDER: S-EPMC6260976 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature