Network assessment of demethylation treatment in melanoma: Differential transcriptome-methylome and antigen profile signatures.

ABSTRACT:

Background

In melanoma, like in other cancers, both genetic alterations and epigenetic underlie the metastatic process. These effects are usually measured by changes in both methylome and transcriptome profiles, whose cross-correlation remains uncertain. We aimed to assess at systems scale the significance of epigenetic treatment in melanoma cells with different metastatic potential.

Methods and findings

Treatment by DAC demethylation with 5-Aza-2'-deoxycytidine of two melanoma cell lines endowed with different metastatic potential, SKMEL-2 and HS294T, was performed and high-throughput coupled RNA-Seq and RRBS-Seq experiments delivered differential profiles (DiP) of both transcriptomes and methylomes. Methylation levels measured at both TSS and gene body were studied to insp

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC6261551 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature