Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins.

ABSTRACT: The discovery and use of fluorescent proteins revolutionized cell biology by allowing the visualization of proteins in living cells. Advances in fluorescent proteins, primarily through genetic engineering, have enabled more advanced analyses, including Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) and the development of genetically encoded fluorescent biosensors. These fluorescence protein-based sensors are highly effective in cells grown in monolayer cultures. However, it is often desirable to use more complex models including tissue explants, organoids, xenografts, and whole animals. These types of samples have poor light penetration owing to high scattering and absorption of light by tissue. Far-red light with a wavelength between 650-900nm

SUBMITTER: Canty L 

PROVIDER: S-EPMC6261627 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature