Pathogenic Citrulline-Multispecific B Cell Receptor Clades in Rheumatoid Arthritis.

ABSTRACT:

Objective

Anti-citrullinated protein antibodies (ACPAs) have proven highly useful as biomarkers for rheumatoid arthritis (RA). However, composition and functionality of the associated autoreactive B cell repertoire have not been directly assessed. We aimed to selectively investigate citrullinated autoantigen-specific B cell receptors (BCRs) involved in RA and initiate studies on their pathogenicity.

Methods

Blood samples were obtained from patients in a University of Minnesota cohort with ACPA-positive RA (n = 89). Tetramer sets bearing citrullinated filaggrin peptide cfc1 or citrullinated α-enolase peptide were constructed to specifically capture autoreactive B cells from the unaltered, polyclonal repertoire in RA patients. Citrullinated peptide tetramer-bound B cells were

SUBMITTER: Titcombe PJ 

PROVIDER: S-EPMC6261688 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature