Role of Macrophage Migration Inhibitory Factor in Granulomatosis With Polyangiitis.

ABSTRACT:

Objective

To examine the association between macrophage migration inhibitory factor (MIF) promoter polymorphisms and granulomatosis with polyangiitis (GPA) in human subjects, and to assess the role of MIF in a murine model of granulomatous vasculitis.

Methods

The human study involved 1,077 patients with GPA and healthy controls whose serum was genotyped by capillary electrophoresis for the MIF -794 CATT_5-8 promoter microsatellite (rs5844572). MIF promoter, CATT-length-dependent gene expression in response to β-glucan was assessed by gene reporter assays. In mouse studies, granulomatous disease was induced by injection of Candida albicans β-glucan into wild-type (WT) or Mif-knockout (Mif-KO) C57BL/6 mice and C57BL/6 mice transgenically overexpressing Mif in lung ep

SUBMITTER: Sreih AG 

PROVIDER: S-EPMC6261690 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature