The effect of sample size on estimates of genetic differentiation and effective population size for Schistosoma mansoni populations.

ABSTRACT: Eradication or local extinction of the human parasite Schistosoma mansoni is a goal for many control programs. Population genetic analyses are helping to evaluate and guide these efforts, yet what to sample, how to sample and how densely to sample is not well established. We determined the S. mansoni allele frequency profile of nearly all infected inhabitants in two small Brazilian communities and created sub-samples representing 5-50% of all detected human infections (infrapopulations). Samples were selected at random with replacement, and each size class was replicated 100 times. Mean pairwise differentiation for all infrapopulations (Di) and the variance effective population size (Ne) were calculated for each sample. Prior to community-wide treatment, the true mean Di was moderate (0.09

SUBMITTER: Barbosa LM 

PROVIDER: S-EPMC6261692 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature