Bioengineered Norovirus S₆₀ Nanoparticles as a Multifunctional Vaccine Platform.

ABSTRACT: Homotypic interactions of viral capsid proteins are common, driving viral capsid self-formation. By taking advantage of such interactions of the norovirus shell (S) domain that naturally builds the interior shells of norovirus capsids, we have developed a technology to produce 60-valent, icosahedral S₆₀ nanoparticles through the E. coli system. This has been achieved by several modifications to the S domain, including an R₆₉A mutation to destruct an exposed proteinase cleavage site and triple cysteine mutations (V₅₇C/Q₅₈C/S₁₃₆C) to establish inter-S domain disulfide bonds for enhanced inter-S domain interactions. The polyvalent S₆₀ nanoparticle with 60 exposed S domain C-termini offers an ideal platform for antigen presentat

SUBMITTER: Xia M 

PROVIDER: S-EPMC6261714 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature