Phylogenetic analysis of canine distemper viruses isolated from vaccinated dogs in Wuhan.

ABSTRACT: Canine distemper virus (CDV) is an infectious agent that can cause canine distemper (CD), a lethal disease. Immunization is an effective method to control the infection; however, some cases of failed immunization are observed in animal hospitals every year. Therefore, in this study, we conducted phylogenetic analysis of the H gene of isolated CDVs. We first constructed a modified MDCK cell line, which constitutively expressed signaling lymphocyte activation molecule (SLAM), a specific receptor for CDV. The modified cell line was more suitable for propagation of CDV than the original MDCK cell line. Next, 9 CDVs were successfully isolated from 20 dogs with suspected CD-associated diseases. Of these CDV isolates, three were from vaccinated dogs. The analysis indicated that the H gene sequenc

SUBMITTER: Li W 

PROVIDER: S-EPMC6261815 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature