Centromere-associated protein E expresses a novel mRNA isoform in acute lymphoblastic leukemia.

ABSTRACT: The alternative splicing plays an important role to generate protein diversity. Recent studies have shown alterations in alternative splicing, resulting in loss, gain or changes of functions in the resulting protein. Specific products of alternative splicing are known to contribute in cancer-related mechanisms, such as angiogenesis, migration, adhesion and cell proliferation, among others. We using high-density microarrays reported a CENP-E as a one of significant transcript expressed and potentially is alternatively spliced in cancer. We focus in validate alternative splicing of CENP-E transcript using RT-PCR and sequencing in different cancer cell lines. We performed RT-PCR using specific primers designed to delimit the non-reported alternative splicing in CENP-E transcript. Our results

SUBMITTER: Jimenez-Avila CE 

PROVIDER: S-EPMC6261922 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature