The phospholipid PI(3,4)P₂ is an apical identity determinant.

ABSTRACT: Apical-basal polarization is essential for epithelial tissue formation, segregating cortical domains to perform distinct physiological functions. Cortical lipid asymmetry has emerged as a determinant of cell polarization. We report a network of phosphatidylinositol phosphate (PIP)-modifying enzymes, some of which are transcriptionally induced upon embedding epithelial cells in extracellular matrix, and that are essential for apical-basal polarization. Unexpectedly, we find that PI(3,4)P₂ localization and function is distinct from the basolateral determinant PI(3,4,5)P₃. PI(3,4)P₂ localizes to the apical surface, and Rab11a-positive apical recycling endosomes. PI(3,4)P₂ is produced by the 5-phosphatase SHIP1 and Class-II PI3-Kinases to recruit the

SUBMITTER: Roman-Fernandez A 

PROVIDER: S-EPMC6262019 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature