Properties of the Permeability Transition of Pea Stem Mitochondria.

ABSTRACT: In striking analogy with Saccharomyces cerevisiae, etiolated pea stem mitochondria did not show appreciable Ca²⁺ uptake. Only treatment with the ionophore ETH129 (which allows electrophoretic Ca²⁺ equilibration) caused Ca²⁺ uptake followed by increased inner membrane permeability, membrane depolarization and Ca²⁺ release. Like the permeability transition (PT) of mammals, yeast and Drosophila, the PT of pea stem mitochondria was stimulated by diamide and phenylarsine oxide and inhibited by Mg-ADP and Mg-ATP, suggesting a common underlying mechanism; yet, the plant PT also displayed distinctive features: (i) as in mammals it was desensitized by cyclosporin A, which does not affect the PT of yeast and Drosophila; (ii) similarly to S. cerev

SUBMITTER: De Col V 

PROVIDER: S-EPMC6262314 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature