New Pim-1 Kinase Inhibitor From the Co-culture of Two Sponge-Associated Actinomycetes.

ABSTRACT: Saccharomonospora sp. UR22 and Dietzia sp. UR66, two actinomycetes derived from the Red Sea sponge Callyspongia siphonella, were co-cultured and the induced metabolites were monitored by HPLC-DAD and TLC. Saccharomonosporine A (1), a novel brominated oxo-indole alkaloid, convolutamydine F (2) along with other three known induced metabolites (3-5) were isolated from the EtOAc extract of Saccharomonospora sp. UR22 and Dietzia sp. UR66 co-culture. Additionally, axenic culture of Saccharomonospora sp. UR22 led to isolation of six known microbial metabolites (6-11). A kinase inhibition assay results showed that compounds 1 and 3 were potent Pim-1 kinase inhibitors with an IC₅₀ value of 0.3 ± 0.02 and

SUBMITTER: El-Hawary SS 

PROVIDER: S-EPMC6262321 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature