Flux Connections Between Gluconate Pathway, Glycolysis, and Pentose-Phosphate Pathway During Carbohydrate Metabolism in Bacillus megaterium QM B1551.

ABSTRACT: Bacillus megaterium is a bacterium of great importance as a plant-beneficial bacterium in agricultural applications and in industrial bioproduction of proteins. Understanding intracellular processing of carbohydrates in this species is crucial to predicting natural carbon utilization as well as informing strategies in metabolic engineering. Here, we applied stable isotope-assisted metabolomics profiling and metabolic flux analysis to elucidate, at high resolution, the connections of the different catabolic routes for carbohydrate metabolism immediately following substrate uptake in B. megaterium QM B1551. We performed multiple ¹³C tracer experiments to obtain both kinetic and long-term ¹³C profiling of intracellular metabolites. In addition to the direct

SUBMITTER: Wushensky JA 

PROVIDER: S-EPMC6262346 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature