A DNA Methylation-Based Test for Breast Cancer Detection in Circulating Cell-Free DNA.

ABSTRACT: Breast cancer (BrC) is the most frequent neoplasm in women. New biomarkers, including aberrant DNA methylation, may improve BrC management. Herein, we evaluated the detection and prognostic performance of seven genes' promoter methylation (APC, BRCA1, CCND2, FOXA1, PSAT1, RASSF1A and SCGB3A1). Methylation levels were assessed in primary BrC tissues by quantitative methylation-specific polymerase chain reaction (QMSP) and in circulating cell-free DNA (ccfDNA) by multiplex QMSP from two independent cohorts of patients (Cohort #1, n = 137; and Cohort #2, n = 44). Receiver operating characteristic (ROC) curves were constructed, and log-rank test and Cox regression were performed to assess the prognostic value of genes' methylation levels. The gene-panel APC, FOXA1, RASSF1A, SCGB3A1 discriminat

SUBMITTER: Salta S 

PROVIDER: S-EPMC6262630 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature