Concordance between different trophectoderm biopsy sites and the inner cell mass of chromosomal composition measured with a next-generation sequencing platform.

ABSTRACT:

Study question

In PGS, does chromosomal constitution differ among trophectoderm (TE) biopsy sites and between them and the inner cell mass (ICM)?

Summary answer

The ploidy concordance between ICM and TE was independent of whether the biopsy site in the TE was near to or far from the ICM.

What is known already

TE biopsies are considered less harmful to developing embryos than blastomere biopsies. Removal of multi-cellular samples permits high-resolution next-generation sequencing (Veriseq NGS) to detect aneuploidy present in a minority of cells (mosaicism of diploid and aneuploid cells). However, the prevalence of ploidy discrepancies between different TE biopsy sites and the ICM, as well as confined mosaicism (aneuploidy only in a particular area), has not been establ

SUBMITTER: Chuang TH 

PROVIDER: S-EPMC6262631 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature