The role of integrin β1 in the heterogeneity of human embryonic stem cells culture.

ABSTRACT: The maintenance of the pluripotency of human embryonic stem (hES) cells requires special conditions for culturing. These conditions include specific growth factors containing media and extracellular matrix (ECM) or an appropriate substrate for adhesion. Interactions between the cells and ECM are mediated by integrins, which interact with the components of ECM in active conformation. This study focused on the characterisation of the role of integrin β1 in the adhesion, migration and differentiation of hES cells. Blocking integrin β1 abolished the adhesion of hES cells, decreasing their survival and pluripotency. This effect was in part rescued by the inhibition of RhoA signalling with Y-27632. The presence of Y-27632 increased the migration of hES cells and supported their differentiation i

SUBMITTER: Kallas-Kivi A 

PROVIDER: S-EPMC6262870 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature