DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations.

ABSTRACT: Over the last few years, protein-based affinity reagents have proven very helpful in cell and developmental biology. While many of these versatile small proteins can be expressed both in the intracellular and extracellular milieu in cultured cells and in living organisms, they can also be functionalized by fusing them to different protein domains in order to regulate or modulate their target proteins in diverse manners. For example, protein binders have been employed to degrade, trap, localize or enzymatically modify specific target proteins. Whereas binders to many endogenous proteins or small protein tags have been generated, several affinity reagents against fluorescent proteins have also been created and used to manipulate target proteins tagged with the corresponding fluorescent prote

SUBMITTER: Vigano MA 

PROVIDER: S-EPMC6262872 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature