Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter.

ABSTRACT: Despite advances made in understanding the effects of promoter structure on transcriptional activity, limited knowledge exists regarding the role played by chromatin architecture in transcription. Previous work hypothesized that transcription from the bidirectional GAL1/GAL10 promoter is controlled through looping of its UAS region around a nonstandard nucleosome. Here, by editing the GAL1/GAL10 promoter at high resolution, we provide insights into bidirectional expression control. We demonstrate that the first and fourth Gal4 binding sites within the UAS do not functionally contribute to promoter activation. Instead, these sites, along with nearby regulatory regions, contribute to the directional regulation of gene expression. Furthermore, Gal4 binding to the third binding site is critica

SUBMITTER: Elison GL 

PROVIDER: S-EPMC6263159 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature