Rapid Turnover and High Production Rate of Myeloid Cells in Adult Rhesus Macaques with Compensations during Aging.

ABSTRACT: Neutrophils, basophils, and monocytes are continuously produced in bone marrow via myelopoiesis, circulate in blood, and are eventually removed from circulation to maintain homeostasis. To quantitate the kinetics of myeloid cell movement during homeostasis, we applied 5-bromo-2'-deoxyuridine pulse labeling in healthy rhesus macaques (Macaca mulatta) followed by hematology and flow cytometry analyses. Results were applied to a mathematical model, and the blood circulating half-life and daily production, respectively, of each cell type from macaques aged 5-10 y old were calculated for neutrophils (1.63 ± 0.16 d, 1.42 × 10⁹ cells/l/d), basophils (1.78 ± 0.30 d, 5.89 × 10⁶ cells/l/d), and CD14⁺CD16^- classical monocytes (1.01 ± 0.15 d, 3.09 × 1

SUBMITTER: He Z 

PROVIDER: S-EPMC6263173 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature