Significant Sensitivity Improvement for Camera-Based Lateral Flow Immunoassay Readers.

ABSTRACT: Recent developments in smartphone-based strip readers have further improved the performances of lateral flow test kits. Most smartphone cameras encode an unaltered and nonlinear power-law transfer function that maps the light intensity to a pixel value; this poses some limitations for camera-based strip readers. For faint-color test lines which are almost as white such as with nitrocellulose pads, the slope of the transfer function is low. Therefore, it is difficult to differentiate between the faint test lines and the white background. We show that by manually setting the camera exposure time-instead of using the automatic settings-to the high-slope region of the transfer function, the reader's sensitivity can be improved. We found that the sensitivity and the limit of detection of the

SUBMITTER: Saisin L 

PROVIDER: S-EPMC6263405 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature