TargetClone: A multi-sample approach for reconstructing subclonal evolution of tumors.

ABSTRACT: Most tumors are composed of a heterogeneous population of subclones. A more detailed insight into the subclonal evolution of these tumors can be helpful to study progression and treatment response. Problematically, tumor samples are typically very heterogeneous, making deconvolving individual tumor subclones a major challenge. To overcome this limitation, reducing heterogeneity, such as by means of microdissections, coupled with targeted sequencing, is a viable approach. However, computational methods that enable reconstruction of the evolutionary relationships require unbiased read depth measurements, which are commonly challenging to obtain in this setting. We introduce TargetClone, a novel method to reconstruct the subclonal evolution tree of tumors from single-nucleotide polymorphism a

SUBMITTER: Nieboer MM 

PROVIDER: S-EPMC6264523 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature