Non-coding RNA Expression, Function, and Variation during Drosophila Embryogenesis.

ABSTRACT: Long non-coding RNAs (lncRNAs) can often function in the regulation of gene expression during development; however, their generality as essential regulators in developmental processes and organismal phenotypes remains unclear. Here, we performed a tailored investigation of lncRNA expression and function during Drosophila embryogenesis, interrogating multiple stages, tissue specificity, nuclear localization, and genetic backgrounds. Our results almost double the number of annotated lncRNAs expressed at these embryonic stages. lncRNA levels are generally positively correlated with those of their neighboring genes, with little evidence of transcriptional interference. Using fluorescent in situ hybridization, we report the spatiotemporal expression of 15 new lncRNAs, revealing very dynamic tis

SUBMITTER: Schor IE 

PROVIDER: S-EPMC6264527 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature