PNPase is involved in the coordination of mRNA degradation and expression in stationary phase cells of Escherichia coli.

ABSTRACT:

Background

Exoribonucleases are crucial for RNA degradation in Escherichia coli but the roles of RNase R and PNPase and their potential overlap in stationary phase are not well characterized. Here, we used a genome-wide approach to determine how RNase R and PNPase affect the mRNA half-lives in the stationary phase. The genome-wide mRNA half-lives were determined by a dynamic analysis of transcriptomes after transcription arrest. We have combined the analysis of mRNA half-lives with the steady-state concentrations (transcriptome) to provide an integrated overview of the in vivo activity of these exoribonucleases at the genome-scale.

Results

The values of mRNA half-lives demonstrated that the mRNAs are very stable in the stationary phase and that the deletion of RNase R or PNP

SUBMITTER: Dressaire C 

PROVIDER: S-EPMC6264599 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature