Integration of sample preparation and analysis into an optofluidic chip for multi-target disease detection.

ABSTRACT: Detection of molecular biomarkers with high specificity and sensitivity from biological samples requires both sophisticated sample preparation and subsequent analysis. These tasks are often carried out on separate platforms which increases required sample volumes and the risk of errors, sample loss, and contamination. Here, we present an optofluidic platform which combines an optical detection section with single nucleic acid strand sensitivity, and a sample processing unit capable of on-chip, specific extraction and labeling of nucleic acid and protein targets in complex biological matrices. First, on-chip labeling and detection of individual lambda DNA molecules down to concentrations of 8 fM is demonstrated. Subsequently, we demonstrate the simultaneous capture, fluorescence tagging and

SUBMITTER: Meena GG 

PROVIDER: S-EPMC6264894 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature