Retinoic acid and BMP4 cooperate with p63 to alter chromatin dynamics during surface epithelial commitment.

ABSTRACT: Human embryonic stem cell (hESC) differentiation promises advances in regenerative medicine^1-3, yet conversion of hESCs into transplantable cells or tissues remains poorly understood. Using our keratinocyte differentiation system, we employ a multi-dimensional genomics approach to interrogate the contributions of inductive morphogens retinoic acid and bone morphogenetic protein 4 (BMP4) and the epidermal master regulator p63 (encoded by TP63)^4,5 during surface ectoderm commitment. In contrast to other master regulators^6-9, p63 effects major transcriptional changes only after morphogens alter chromatin accessibility, establishing an epigenetic landscape for p63 to modify. p63 distally closes chromatin accessibility and promotes accumulation of H3K27me3 (trim

SUBMITTER: Pattison JM 

PROVIDER: S-EPMC6265075 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature