Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

ABSTRACT: The RNA exosome is a conserved multi-subunit complex essential for processing and degradation of several types of RNAs. Although many of the functions of the RNA exosome are well established, whether the activity of this complex is regulated remains unclear. Here we performed a proteomic analysis of the RNA exosome complex purified from Schizosaccharomyces pombe and identified 39 post-translational modifications (PTMs), including phosphorylation, methylation, and acetylation sites. Interestingly, most of the modifications were identified in Dis3, a catalytic subunit of the RNA exosome, as well as in the exosome-associated RNA helicase, Mtr4. Functional analysis of selected PTM sites using modification-deficient and -mimetic versions of exosome subunits revealed substitutions that affected

SUBMITTER: Telekawa C 

PROVIDER: S-EPMC6265454 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature