Modular 5'-UTR hexamers for context-independent tuning of protein expression in eukaryotes.

ABSTRACT: Functional characterization of regulatory DNA elements in broad genetic contexts is a prerequisite for forward engineering of biological systems. Translation initiation site (TIS) sequences are attractive to use for regulating gene activity and metabolic pathway fluxes because the genetic changes are minimal. However, limited knowledge is available on tuning gene outputs by varying TISs in different genetic and environmental contexts. Here, we created TIS hexamer libraries in baker's yeast Saccharomyces cerevisiae directly 5' end of a reporter gene in various promoter contexts and measured gene activity distributions for each library. Next, selected TIS sequences, resulted in almost 10-fold changes in reporter outputs, were experimentally characterized in various environmental and genetic

SUBMITTER: Petersen SD 

PROVIDER: S-EPMC6265478 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature