Microfluidic Analyzer Enabling Quantitative Measurements of Specific Intracellular Proteins at the Single-Cell Level.

ABSTRACT: This paper presents a microfluidic instrument capable of quantifying single-cell specific intracellular proteins, which are composed of three functioning modules and two software platforms. Under the control of a LabVIEW platform, a pressure module flushed cells stained with fluorescent antibodies through a microfluidic module with fluorescent intensities quantified by a fluorescent module and translated into the numbers of specific intracellular proteins at the single-cell level using a MATLAB platform. Detection ranges and resolutions of the analyzer were characterized as 896.78⁻6.78 × 10⁵ and 334.60 nM for Alexa 488, 314.60⁻2.11 × 10⁵ and 153.98 nM for FITC, and 77.03⁻5.24 × 10⁴ and 37.17 nM for FITC-labelled anti-beta-actin antibodies. As a demonstration, the numbers of single-cell bet

SUBMITTER: Liu L 

PROVIDER: S-EPMC6265747 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature