Nuclear Magnetic Resonance seq (NMRseq): A New Approach to Peptide Sequence Tags.

ABSTRACT: Structural analysis of peptides with nuclear magnetic resonance (NMR) spectroscopy generally relies on knowledge of the primary sequence to enable assignment of the resonances prior to determination of the three-dimensional structure. Resonance assignment without knowledge of the sequence is complicated by redundancy in amino acid type, making complete de novo sequencing using NMR spectroscopy unlikely to be feasible. Despite this redundancy, we show here that NMR spectroscopy can be used to identify short sequence tags that can be used to elucidate full-length peptide sequences via database searching. In the current study, we have used this approach to identify conotoxins from the venom of the cone snail Conus geographus and determined the three-dimensional structure of a member of the I3

SUBMITTER: Wilson D 

PROVIDER: S-EPMC6266860 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature