Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry.

ABSTRACT:

Background

Astrocytes are the most abundant cells in the central nervous system and are responsible for a wide range of functions critical to normal neuronal development, synapse formation, blood-brain barrier regulation, and brain homeostasis. They are also actively involved in initiating and perpetuating neuroinflammatory responses. However, information about their proteomic phenotypes under inflammation is currently limited.

Method

Data-independent acquisition mass spectrometry was applied to extensively characterize the profile of more than 4000 proteins in immortalized human fetal astrocytes under distinct inflammatory conditions induced by TNF, IL-1β, and LPS, while multiplex immunoassay-based screening was used to quantify a wide range of cytokines released under thes

SUBMITTER: Dozio V 

PROVIDER: S-EPMC6267034 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature