A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes.

ABSTRACT:

Background

The transmission of malaria to mosquitoes depends on the presence of gametocytes that circulate in the peripheral blood of infected human hosts. Sensitive estimates of the densities of female gametocytes (FG) and male gametocytes (MG) may allow the prediction of infectivity to mosquitoes and thus a molecular estimate of the human infectious reservoir for transmission.

Methods

A novel multiplex qRT-PCR assay with intron-spanning primers was developed for the parallel quantification of FG and MG. CCp4 (PF3D7_0903800) transcripts specific for FG and PfMGET (PF3D7_1469900) transcripts specific for MG were quantified in total nucleic acids. The assay was validated on sex-sorted gametocytes from culture material and on samples from clinical trials with gametocytocidal d

SUBMITTER: Meerstein-Kessel L 

PROVIDER: S-EPMC6267050 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature