Differential protein expression in human knee articular cartilage and medial meniscus using two different proteomic methods: a pilot analysis.

ABSTRACT:

Background

Proteomics is an emerging field in the study of joint disease. Our two aims with this pilot analysis were to compare healthy human knee articular cartilage with meniscus, two tissues both known to become affected in the osteoarthritic disease process, and to compare two mass spectrometry (MS)-based methods: data-dependent acquisition (DDA) and data-independent acquisition (DIA).

Methods

Healthy knee articular cartilage taken from the medial tibial condyle and medial meniscus samples taken from the body region were obtained from three adult forensic medicine cases. Proteins were extracted from tissue pieces and prepared for MS analysis. Each sample was subjected to liquid chromatography (LC)-MS/MS analysis using an Orbitrap mass spectrometer, and run in both DDA an

SUBMITTER: Folkesson E 

PROVIDER: S-EPMC6267052 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature