Proteomic profiling of senescent human diploid fibroblasts treated with gamma-tocotrienol.

ABSTRACT:

Background

Replicative senescence of human diploid fibroblasts (HDFs) has been used as a model to study mechanisms of cellular aging. Gamma-tocotrienol (γT3) is one of the members of vitamin E family which has been shown to increase proliferation of senescent HDFs. However, the modulation of protein expressions by γT3 in senescent HDFs remains to be elucidated. Therefore, this study aimed to determine the differentially expressed proteins (DEPs) in young and senescent HDFs; and in vehicle- and γT3-treated senescent HDFs using label-free quantitative proteomics.

Methods

Whole proteins were extracted and digested in-gel with trypsin. Peptides were detected by Orbitrap liquid chromatography mass spectrometry. Mass spectra were identified and quantitated by MaxQuant software. Th

SUBMITTER: Tan JK 

PROVIDER: S-EPMC6267793 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature