Effects of ploidy variation on promoter DNA methylation and gene expression in rice (Oryza sativa L.).

ABSTRACT:

Background

Polyploidy, or whole-genome duplication (WGD) promotes genetic diversification in plants. However, whether WGD is accompanied by epigenetic regulation especially DNA methylation remains yet elusive. Methylation of different region in genomic DNA play discrete role in gene regulation and developmental processes in plants.

Results

In our study, we used an apomictic rice line (SARII-628) that produces twin seedlings of different ploidy for methylated DNA immunoprecipitation sequencing (MeDIP-seq). We compared the level of methylation and mRNA expression in three different (CG, CHG, and CHH) sequence contexts of promoter region among haploid (1X), diploid (2X), and triploid (3X) seedling. We used MeDIP-Seq analysis of 14 genes to investigate whole genome DNA methylati

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6267922 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature