Cloning, phylogenetic analysis and 3D modeling of a putative lysosomal acid lipase from the camel, Camelus dromedarius.

ABSTRACT: Acid lipase belongs to a family of enzymes that is mainly present in lysosomes of different organs and the stomach. It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first time the full coding sequence of camel's lysosomal acid lipase, cLIPA using RT-PCR technique (Genbank accession numbers JF803951 and AEG75815, for the nucleotide and aminoacid sequences respectively). The cDNA sequencing revealed an open reading frame of 1,197 nucleotides that encodes a protein of 399 aminoacids which was similar to that from other related mammalian species. Bioinformatic analysis was used to determine the aminoacid sequence, 3D structure and phylogeny of cLIPA. Bioinformatics analysis suggested the molecular weight of the tran

SUBMITTER: Ataya FS 

PROVIDER: S-EPMC6268779 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature